Question 1

Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium

Accepted Answer

A)  Agrobacterium tumefaciens.
B)  Bacillus thuringiensis.
C)  Herpes simplex.
D)  Pseudomonas.
E)  tobacco mosaic.F) A) and B)
G) B) and E)

Question 2

Restriction enzymes are

Accepted Answer

A)  proteases.
B)  lipases.
C)  endonucleases.
D)  exonucleases.E) A) and D)
F) A) and C)

Question 3

Bacterial plasmids and artificial chromosomes are used as ________ to insert foreign DNA into host cells and create recombinant genomes.

Accepted Answer

A)  clones
B)  vectors
C)  endonucleases
D)  subunitsE) B) and C)
F) None of the above

Question 4

Select the experiments that can be characterized as reverse genetics: (check all that apply)

Accepted Answer

A)  Generation and analysis of a knockout mouse
B)  Random mutagenesis of an organism,then selecting mutants by phenotype
C)  RNA interference of a gene in C.elegans
D)  Using reverse transcriptase to create a cDNA libraryE) B) and C)
F) A) and C)

Question 5

Both DNA replication and PCR require: (check all that apply)

Accepted Answer

A)  DNA polymerase.
B)  DNA helicase.
C)  a DNA template.
D)  a thermocycler machine.
E)  plasmid DNA.F) B) and D)
G) C) and E)

Question 6

Which component of the PCR mix do researchers manipulate to induce a site-specific mutation?

Accepted Answer

A)  Primers
B)  Taq polymerase
C)  DNA template
D)  Reverse transcriptaseE) None of the above
F) A) and D)

Question 7

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,using molecular cloning techniques?

Accepted Answer

A)  DNA fingerprint data
B)  Sequence of PCR primers
C)  Data from cloning of Dolly the sheep
D)  Restriction maps of the plasmidsE) B) and D)
F) None of the above

Question 8

The most common way to follow bacterial transformation with a plasmid is by

Accepted Answer

A)  manufacturing the bacterial protein.
B)  conferring antibiotic resistance.
C)  separating the altered cell surface.
D)  making the cells immortal.
E)  altering the physical appearance of the cells.F) C) and D)
G) C) and E)

Exam 17: Biotechnology

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,using molecular cloning techniques?

Correct Answer
verified

Restriction enzymes are

Correct Answer
verified

Bacterial plasmids and artificial chromosomes are used as ________ to insert foreign DNA into host cells and create recombinant genomes.

Correct Answer
verified

Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium

Correct Answer
verified

Both DNA replication and PCR require: (check all that apply)

Correct Answer
verified

Which component of the PCR mix do researchers manipulate to induce a site-specific mutation?

Correct Answer
verified

The most common way to follow bacterial transformation with a plasmid is by

Correct Answer
verified

Select the experiments that can be characterized as reverse genetics: (check all that apply)

Correct Answer
verified

Exam 17: Biotechnology

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,using molecular cloning techniques?

Correct AnswerverifiedShow Answer

Restriction enzymes are

Correct AnswerverifiedShow Answer

Bacterial plasmids and artificial chromosomes are used as ________ to insert foreign DNA into host cells and create recombinant genomes.

Correct AnswerverifiedShow Answer

Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium

Correct AnswerverifiedShow Answer

Both DNA replication and PCR require: (check all that apply)

Correct AnswerverifiedShow Answer

Which component of the PCR mix do researchers manipulate to induce a site-specific mutation?

Correct AnswerverifiedShow Answer

The most common way to follow bacterial transformation with a plasmid is by

Correct AnswerverifiedShow Answer

Select the experiments that can be characterized as reverse genetics: (check all that apply)

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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