Question 1

This is the type of microtome that is used for cutting sections for Electron microscopy.

Accepted Answer

A)  Rotary microtome
B)  Rocking microtome
C)  Ultrathin microtome
D)  Freezing microtomeE) A) and C)
F) B) and D)

Question 2

In cutting the tissue block, a hole is observed in the section. What do you think is the reason for this?

Accepted Answer

A)  Sections are too thin
B)  Incomplete dehydration, clearing or infiltration of tissue with wax
C)  Sections are too thick
D)  Dirt was formed in the embedding mediumE) None of the above
F) A) and B)

Question 3

Don recalls that the following items are also needed during microtomy, aside from:

Accepted Answer

A)  Waterbath
B)  Clean slides
C)  Permanent marker
D)  Drying ovenE) B) and C)
F) C) and D)

Question 4

What type of microtome is used for cutting celloidin embedded sections?

Accepted Answer

A)  Rotary microtome
B)  Rocking microtome
C)  Base-sledge microtome
D)  Freezing microtomeE) B) and C)
F) A) and D)

Question 5

What is the usual range of thickness in a cut of tissue for routine histologic procedures?

Accepted Answer

A)  0) 5 - 1.0 microns
B)  1) 0 - 2.0 microns
C)  3) 0 - 4.5 microns
D)  4) 0 - 6.0 micronsE) B) and D)
F) A) and B) D

Question 6

Chino understands that the following are precautions to observe during stropping, except:

Accepted Answer

A)  Vegetable oil should be applied on the surface of the strop.
B)  Pressure applied during the initial strokes of stropping should be relatively light and gentle.
C)  Stopping in a quick and fast manner should be avoided.
D)  Mineral oil is never used since it damages the leather.E) A) and B)
F) B) and C) A

Question 7

What type of microtome is used for cutting un-embedded tissue sections?

Accepted Answer

A)  Rotary microtome
B)  Rocking microtome
C)  Sliding microtome
D)  Freezing microtomeE) A) and C)
F) C) and D)

Question 8

This is the specific type of microtome that was invented by Paldwell Trefall in 1881?

Accepted Answer

A)  Rotary microtome
B)  Sliding microtome
C)  Rocking microtome
D)  Freezing microtomeE) B) and C)
F) A) and B)

Question 9

In what angle is the knife usually tilted in the microtome?

Accepted Answer

A)  35-45 degrees
B)  25-35 degrees
C)  15-25 degrees
D)  0 -15 degreesE) B) and D)
F) All of the above

Question 10

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?

Accepted Answer

A)  Biconcave knife
B)  Plane-Concave knife
C)  Plane-wedge knife
D)  None of the aboveE) A) and C)
F) B) and C)

Question 11

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?

Accepted Answer

A)  Biconcave knife
B)  Plane-Concave knife
C)  Plane-wedge knife
D)  None of the aboveE) A) and B)
F) A) and C)

Question 12

Bryan must take note that one of the following is true about the precautions during honing.

Accepted Answer

A)  The hone should be of a certain length at 8 cm by 3 cm to allow the entire knife edge to be sharpened in one stroke.
B)  The pressure exerted on the knife should be kept at a steady and gentle manner to avoid it from rocking.
C)  The hone shall always be lubricated with cold, soapy water before its usage.
D)  The fluid used for washing must be flowing in a slow motion so that chips of metal are effectively removed.E) None of the above
F) B) and C)

Question 13

All of the above statements are true about the freezing microtome, except:

Accepted Answer

A)  The freezing microtome was invented by Minot in 1848.
B)  The freezing microtome was invented by Queckett in 1848.
C)  The freezing microtome's stage for block holder is hallow and perforated around its perimeter.
D)  The freezing microtome can cut un-embedded tissue sections.E) A) and D)
F) A) and C)

Question 14

What type of microtome is used for cutting serial sections of large blocks of paraffin-embedded tissue?

Accepted Answer

A)  Rotary microtome
B)  Rocking microtome
C)  Sliding microtome
D)  Freezing microtomeE) A) and B)
F) A) and C)

Question 15

In the cold knife procedure, there is a term called as "Dew Line". What do you think is the meaning of this?

Accepted Answer

A)  Different temperatures between the tissue and the knife
B)  The point at which sections may then be cut at 10 microns of thickness
C)  Optimum condition for sectioning
D)  Freezing the specimenE) None of the above
F) C) and D)

Question 16

Which of the following sharpening stones or hones gives a more polishing effect?

Accepted Answer

A)  Belgium Yellow stone
B)  Arkansas stone
C)  Fine Carborundum stone
D)  Niether A or BE) C) and D)
F) B) and D)

Question 17

All of the following statements are true about why lengthwise vertical scratches are seen on the sections, except:

Accepted Answer

A)  The knife is sharp
B)  Dirty embedding medium
C)  The tilt of the knife is too great
D)  Nicks on the edge of the knifeE) All of the above
F) C) and D)

Question 18

The machine or instrument used for cutting sections is known as the:

Accepted Answer

A)  Knife
B)  Block holder
C)  Microtome
D)  Ratchet feed wheelE) A) and B)
F) All of the above

Question 19

This microtome was developed by Adams in 1789.

Accepted Answer

A)  Rotary microtome
B)  Sliding microtome
C)  Rocking microtome
D)  Freezing microtomeE) A) and C)
F) A) and B)

Question 20

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?

Accepted Answer

A)  Stropping
B)  Honing
C)  Sharpening
D)  CuttingE) A) and B)
F) A) and C) A

Exam 3: Microtomy

This is the type of microtome that is used for cutting sections for Electron microscopy.

Correct AnswerverifiedShow Answer

In cutting the tissue block, a hole is observed in the section. What do you think is the reason for this?

Correct AnswerverifiedShow Answer

Don recalls that the following items are also needed during microtomy, aside from:

Correct AnswerverifiedShow Answer

What type of microtome is used for cutting celloidin embedded sections?

Correct AnswerverifiedShow Answer

What is the usual range of thickness in a cut of tissue for routine histologic procedures?

Correct AnswerverifiedD

Chino understands that the following are precautions to observe during stropping, except:

Correct AnswerverifiedA

What type of microtome is used for cutting un-embedded tissue sections?

Correct AnswerverifiedShow Answer

This is the specific type of microtome that was invented by Paldwell Trefall in 1881?

Correct AnswerverifiedShow Answer

In what angle is the knife usually tilted in the microtome?

Correct AnswerverifiedShow Answer

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?

Correct AnswerverifiedShow Answer

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?

Correct AnswerverifiedShow Answer

Bryan must take note that one of the following is true about the precautions during honing.

Correct AnswerverifiedShow Answer

All of the above statements are true about the freezing microtome, except:

Correct AnswerverifiedShow Answer

What type of microtome is used for cutting serial sections of large blocks of paraffin-embedded tissue?

Correct AnswerverifiedShow Answer

In the cold knife procedure, there is a term called as "Dew Line". What do you think is the meaning of this?

Correct AnswerverifiedShow Answer

Which of the following sharpening stones or hones gives a more polishing effect?

Correct AnswerverifiedShow Answer

All of the following statements are true about why lengthwise vertical scratches are seen on the sections, except:

Correct AnswerverifiedShow Answer

The machine or instrument used for cutting sections is known as the:

Correct AnswerverifiedShow Answer

This microtome was developed by Adams in 1789.

Correct AnswerverifiedShow Answer

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?

Correct AnswerverifiedA

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D

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A

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A