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Why is it necessary to "starve" the donor cells prior to reproductive cloning?


A) to force the cells into G0 stage so that the nuclei removed from them will respond to cytoplasmic growth signals
B) to force the enucleated egg cell into G0 stage so that it would accept the donor nucleus
C) to lengthen the telomeres in the donor nucleus
D) to make the nucleus "start over" in the S stage so that the nucleus will be diploid
E) to make the nucleus "start over" in the S stage so that the nucleus will be haploid

F) A) and C)
G) B) and D)

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Therapeutic cloning


A) can produce clones of adult individuals.
B) can be used to produce superior farm animals.
C) can produce specialized cells to treat human disease.
D) can be used to create new species of plants.
E) can produce embryonic stem cells.

F) B) and D)
G) A) and C)

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Which gene on chromosome 22 is believed to have made a difference in the evolution of humans compared to that of chimpanzees? Rev: 02_07_2014_QC_44610


A) The gene for proper speech development.
B) The gene for smell.
C) The gene for hearing.
D) The gene for blood type.
E) The gene for social behaviors.

F) B) and E)
G) C) and D)

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Which of the following sequences of events in the production of recombinant DNA are in the correct order?


A) cut DNA with restriction enzymes, mix DNA with plasmid DNA, add DNA ligase
B) mix DNA with plasmid DNA, cut with restriction enzymes, add DNA ligase
C) add DNA ligase, cut DNA with restriction enzymes, mix DNA with plasmid DNA
D) mix DNA with plasmid DNA, add DNA ligase, cut with restriction enzymes
E) cut with restriction enzymes, add DNA ligase, mix DNA with plasmid DNA

F) C) and E)
G) All of the above

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_____ is the study of the structure and function of cellular proteins and their interactions; _______ may be employed to use computer algorithms to aid this process. Rev: 02_07_2014_QC_44566


A) Proteomics; bioinformatics
B) Comparative genomics; bioinformatics
C) Genomics; proteomics
D) Proteomics; comparative genomics
E) Comparative genomics; proteomics

F) B) and D)
G) C) and D)

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Transgenic plants may have genes from animals inserted into their DNA.

A) True
B) False

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How can comparative genomics assist in the treatment of HIV?


A) The genome of HIV can now be compared to the human genome to find similarities between the two.
B) Knowing the genome of HIV allows for the manufacture of designer drugs to treat specific strains.
C) Understanding the genetic code of HIV allows scientists to replicate it in the laboratory.
D) Understanding the evolution of HIV in an individual will help scientists understand how the virus responds to different drug regimes and will lead to better treatments.
E) Knowing the proteins produced by HIV will allow the development of drugs to mimic those proteins.

F) A) and E)
G) A) and B)

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Specialized cells differ from one another because


A) they only express certain genes.
B) they express all genes.
C) they turn off gene expression once specialized.
D) they may re-enter the cell cycle at any time.
E) they do not express housekeeping genes.

F) A) and B)
G) A) and D)

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Organisms that have had foreign genes inserted into them are called transgenic organisms.

A) True
B) False

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True

Which of the following is not an application of polymerase chain reaction?


A) production of many copies of an unknown piece of DNA
B) making copies of a foreign gene for use in making recombinant DNA
C) DNA fingerprinting of a crime scene suspect
D) amplifying mitochondrial DNA sequences
E) linking two DNA molecules together

F) A) and C)
G) A) and E)

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E

In order to clone adult animals,scientists typically begin with


A) an isolated sperm cell.
B) an enucleated egg.
C) an enucleated liver cell.
D) a red blood cell.
E) an enucleated sperm cell.

F) A) and B)
G) B) and E)

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Which of the following is not an application of polymerase chain reaction?


A) production of many copies of an unknown piece of DNA
B) making copies of a foreign gene for use in making recombinant DNA
C) DNA fingerprinting of a crime suspect
D) linking two DNA molecules together
E) amplifying mitochondrial DNA sequences

F) B) and E)
G) B) and C)

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D

Which type of DNA is used during DNA fingerprinting?


A) noncoding sections of DNA that consist of two to five bases repeated over and over again
B) coding sections of DNA that consist of two to five bases repeated over and over again
C) noncoding sections of DNA that consist of the same base repeated over and over again
D) noncoding sections of RNA that consist of two to five bases repeated over and over again
E) noncoding sections of DNA that only consist of adenine and guanine

F) All of the above
G) C) and D)

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During recombinant DNA technology what structure acts as a carrier for the foreign DNA?


A) vector
B) restriction enzyme
C) DNA ligase
D) polymerase chain reaction
E) DNA helicase

F) A) and C)
G) C) and D)

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The steps involved in a single PCR cycle,in the correct order,are


A) cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
B) heat to denature DNA strands, cool to allow primer to anneal and to allow DNA polymerase to copy DNA.
C) heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
D) cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
E) cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.

F) A) and E)
G) None of the above

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When DNA fingerprinting is performed,why might an individual possess two different numbers of repeats for the same chromosomal region?


A) Intergenic DNA may vary from one cell to another in the same individual.
B) The individual has different numbers of the same repeated sequence on each homologous chromosome.
C) The sample is contaminated with the DNA of another individual.
D) Each sister chromatid of a chromosome may contain different numbers of repeats.
E) It is not possible for this to occur.

F) A) and B)
G) D) and E)

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How does DNA fingerprinting distinguish one individual from another?


A) DNA fingerprinting makes use of noncoding sections of DNA that consist of two to five bases repeated over and over again which looks for the number of times such a sequence is repeated.
B) DNA fingerprinting makes use of coding sections of DNA that consist of two to five bases repeated over and over again which looks for the number of times such a sequence is repeated.
C) DNA fingerprinting makes use of noncoding sections of DNA that consist of a single base repeated over and over again which looks for the number of times such a sequence is repeated.
D) DNA fingerprinting makes use of noncoding sections of RNA that consist of two to five bases repeated over and over again which looks for the number of times such a sequence is repeated.
E) DNA fingerprinting makes use of coding sections of RNA that consist of two to five bases repeated over and over again which looks for the number of times such a sequence is repeated.

F) B) and D)
G) B) and C)

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Which organisms have been modified to produce insulin,blood clotting factor VIII,and human growth hormone?


A) bacteria
B) goats
C) cows
D) corn
E) soy beans

F) A) and E)
G) A) and C)

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Cells from the root of a carrot may be used to generate an entire carrot plant.This illustrates that


A) only cells of the root contain the plant's genes.
B) every cell of the root contains all of the plant's genes.
C) plants are easier to clone than animals.
D) carrots may be used for therapeutic cloning.
E) a carrot's roots contain all of the tissues of an adult plant.

F) A) and D)
G) None of the above

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Based on genomic studies,when did a change in gene sequences occur that lead to a change in the way that the brain processes speech?


A) between 1 and 6 million years ago
B) between 4 and 9 million years ago
C) between 10,000 and 100,000 years ago
D) approximately 25,000 years ago
E) approximately 12,000 years ago

F) C) and D)
G) B) and E)

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